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e coli strains cft073  (ATCC)


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    Structured Review

    ATCC e coli strains cft073
    E Coli Strains Cft073, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+cft073+genomic+dna/Quantitative+Genomic+DNA+from+Escherichia+coli+strain+CFT073/pm39089966-116-17-21
    Average 93 stars, based on 89 article reviews
    e coli strains cft073 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Colibactin assembly line enzymes use S -adenosylmethionine to build a cyclopropane ring
    Article Snippet: Linearized pTrcHisA vector was PCR amplified from pTrcHisA vector (Invitrogen) using primers shown in . .. Full-length clbH , truncated clbH ( clbH-C-A2-PCP ), and two adjacent genes clbH and clbI ( clbH-clbI ) were PCR amplified from E. coli CFT073 genomic DNA (ATCC) using primers shown in . .. PCR reactions (20 μL) contained 10 μL Q5 High-Fidelity 2× Master Mix (New England Biolabs), 1 ng of DNA template, and 500 pmoles of each primer.

    Article Title: Colibactin assembly line enzymes use S -adenosylmethionine to build a cyclopropane ring
    Article Snippet: Protein concentrations were determined by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo). .. The cloning, overexpression, and purification of ClbN, ClbC, ClbI, and Sfp were reported previously., , The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection [ATCC]) using the primers shown in . .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England Biolabs), 2 ng of DNA template, and 500 pmoles of each primer.

    Article Title: Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring.
    Article Snippet: Linearized pTrcHisA vector was PCR amplified from pTrcHisA vector (Invitrogen) using primers shown in Supplementary Table 4. .. Full-length clbH, truncated clbH (clbH-C-A2-PCP), and two adjacent genes clbH and clbI (clbH-clbI) were PCR amplified from E. coli CFT073 genomic DNA (ATCC) using primers shown in Supplementary Table 4. .. PCR reactions (20 μL) contained 10 μL Q5 HighFidelity 2× Master Mix (New England BioLabs), 1 ng of DNA template, and 500 pmoles of each primer.

    Article Title: Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring.
    Article Snippet: .. The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection (ATCC)) using the primers shown in Supplementary Table 1. .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England BioLabs), 2 ng of DNA template, and 500 pmoles of each primer.

    Article Title: The reactivity of an unusual amidase may explain colibactin’s DNA cross-linking activity
    Article Snippet: Linearized S4 pTrcHisA vector was PCR amplified from pTrcHisA vector (Thermo Fisher Scientific) using primers shown in Supplementary Table 2. .. Full-length clbL was PCR amplified from E. coli CFT073 genomic DNA (purchased from ATCC) using primers shown in Supplementary Table 2. ..

    Article Title: Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring
    Article Snippet: Linearized pTrcHisA vector was PCR amplified from pTrcHisA vector (Invitrogen) using primers shown in Supplementary Table 4. .. Full-length clbH, truncated clbH (clbH-C-A2-PCP), and two adjacent genes clbH and clbI (clbH-clbI) were PCR amplified from E. coli CFT073 genomic DNA (ATCC) using primers shown in Supplementary Table 4. .. PCR reactions (20 μL) contained 10 μL Q5 HighFidelity 2× Master Mix (New England BioLabs), 1 ng of DNA template, and 500 pmoles of each primer.

    Article Title: Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring
    Article Snippet: .. The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection (ATCC)) using the primers shown in Supplementary Table 1. .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England BioLabs), 2 ng of DNA template, and 500 pmoles of each primer.

    Amplification:

    Article Title: Colibactin assembly line enzymes use S -adenosylmethionine to build a cyclopropane ring
    Article Snippet: Linearized pTrcHisA vector was PCR amplified from pTrcHisA vector (Invitrogen) using primers shown in . .. Full-length clbH , truncated clbH ( clbH-C-A2-PCP ), and two adjacent genes clbH and clbI ( clbH-clbI ) were PCR amplified from E. coli CFT073 genomic DNA (ATCC) using primers shown in . .. PCR reactions (20 μL) contained 10 μL Q5 High-Fidelity 2× Master Mix (New England Biolabs), 1 ng of DNA template, and 500 pmoles of each primer.

    Article Title: Colibactin assembly line enzymes use S -adenosylmethionine to build a cyclopropane ring
    Article Snippet: Protein concentrations were determined by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo). .. The cloning, overexpression, and purification of ClbN, ClbC, ClbI, and Sfp were reported previously., , The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection [ATCC]) using the primers shown in . .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England Biolabs), 2 ng of DNA template, and 500 pmoles of each primer.

    Article Title: Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring.
    Article Snippet: Linearized pTrcHisA vector was PCR amplified from pTrcHisA vector (Invitrogen) using primers shown in Supplementary Table 4. .. Full-length clbH, truncated clbH (clbH-C-A2-PCP), and two adjacent genes clbH and clbI (clbH-clbI) were PCR amplified from E. coli CFT073 genomic DNA (ATCC) using primers shown in Supplementary Table 4. .. PCR reactions (20 μL) contained 10 μL Q5 HighFidelity 2× Master Mix (New England BioLabs), 1 ng of DNA template, and 500 pmoles of each primer.

    Article Title: Investigating the Physiological Roles of Low-Efficiency d -Mannonate and d -Gluconate Dehydratases in the Enolase Superfamily: Pathways for the Catabolism of l -Gulonate and l -Idonate
    Article Snippet: The proteins were purified from 1 L cultures using a chelating Sepharose Fast Flow (Amersham Biosciences) column charged with Ni 2+ as previously described, concentrated ( He GulDH to 15 mg/mL, Se IdoDH to 8.1 mg/mL, and Se GlcDH to 4.7 mg/mL), flash-frozen using liquid nitrogen, and stored at −80 °C prior to use. .. The gene encoding RspB, the l -idonate 5-dehydrogenase from E. coli CFT073 (Uniprot entry Q8FHC8), was amplified from E. coli CFT073 genomic DNA (ATCC 700928) using RspB_FOR and RspB_REV primers (Table S1 of the ). .. The mixture for the polymerase chain reaction (PCR) (30 μL) contained 50 ng of template, 1 mM MgCl 2 , 1× Pfx Amp Buffer, 0.33 mM dNTP, primers (0.33 μM each), and 1.25 units of Pfx polymerase (Invitrogen Platinum Pfx DNA Polymerase kit).

    Article Title: Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring.
    Article Snippet: .. The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection (ATCC)) using the primers shown in Supplementary Table 1. .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England BioLabs), 2 ng of DNA template, and 500 pmoles of each primer.

    Article Title: The reactivity of an unusual amidase may explain colibactin’s DNA cross-linking activity
    Article Snippet: Linearized S4 pTrcHisA vector was PCR amplified from pTrcHisA vector (Thermo Fisher Scientific) using primers shown in Supplementary Table 2. .. Full-length clbL was PCR amplified from E. coli CFT073 genomic DNA (purchased from ATCC) using primers shown in Supplementary Table 2. ..

    Article Title: Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring
    Article Snippet: Linearized pTrcHisA vector was PCR amplified from pTrcHisA vector (Invitrogen) using primers shown in Supplementary Table 4. .. Full-length clbH, truncated clbH (clbH-C-A2-PCP), and two adjacent genes clbH and clbI (clbH-clbI) were PCR amplified from E. coli CFT073 genomic DNA (ATCC) using primers shown in Supplementary Table 4. .. PCR reactions (20 μL) contained 10 μL Q5 HighFidelity 2× Master Mix (New England BioLabs), 1 ng of DNA template, and 500 pmoles of each primer.

    Article Title: Colibactin assembly line enzymes use S-adenosylmethionine to build a cyclopropane ring
    Article Snippet: .. The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection (ATCC)) using the primers shown in Supplementary Table 1. .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England BioLabs), 2 ng of DNA template, and 500 pmoles of each primer.

    Cloning:

    Article Title: Colibactin assembly line enzymes use S -adenosylmethionine to build a cyclopropane ring
    Article Snippet: Protein concentrations were determined by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo). .. The cloning, overexpression, and purification of ClbN, ClbC, ClbI, and Sfp were reported previously., , The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection [ATCC]) using the primers shown in . .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England Biolabs), 2 ng of DNA template, and 500 pmoles of each primer.

    Over Expression:

    Article Title: Colibactin assembly line enzymes use S -adenosylmethionine to build a cyclopropane ring
    Article Snippet: Protein concentrations were determined by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo). .. The cloning, overexpression, and purification of ClbN, ClbC, ClbI, and Sfp were reported previously., , The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection [ATCC]) using the primers shown in . .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England Biolabs), 2 ng of DNA template, and 500 pmoles of each primer.

    Purification:

    Article Title: Colibactin assembly line enzymes use S -adenosylmethionine to build a cyclopropane ring
    Article Snippet: Protein concentrations were determined by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo). .. The cloning, overexpression, and purification of ClbN, ClbC, ClbI, and Sfp were reported previously., , The genes clbB and clbH were PCR amplified from E. coli CFT073 genomic DNA (purchased from the American Type Culture Collection [ATCC]) using the primers shown in . .. A typical PCR reaction (50 μL) contained 25 μL of Phusion High-Fidelity PCR Master Mix (New England Biolabs), 2 ng of DNA template, and 500 pmoles of each primer.



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